SPECIFICATIONS FOR AMBICIN® L (RECOMBINANT LYSOSTAPHIN)
|
| Gene: |
Lysostaphin gene from Staphylococcus simulans, Genbank accession numbers M15686 and I00376 |
| Expression organism: |
E. coli |
| Activity assay: |
Determined by the decrease in turbidity of a suspension of heat-killed Staphylococcus aureus 8325-4
at pH 8.0, 30° C (AMBI Protocol TP-031). Specific activity is measured relative to an AMBI lysostaphin standard with a defined activity of 4,000 U/mg |
| Storage conditions: |
| Long-term: |
Store in sealed container with desiccant at -20° C.
|
| Solution: |
Stable for at least 6 months when stored at 4°C as 10 mg/ml solution in 20 mM sodium acetate,
pH 4.5, in a polypropylene container. |
|
| Method of analysis: |
Reverse phase HPLC monitored at 210 nm (AMBI Protocol TP-112.M) |
| Peptide purity: |
>90% (reverse phase HPLC) |
| mg protein/mg solid: |
>0.9 (Absorbance at 280 nm; extinction coefficient = 2.02 AU/mg/ml) |
| Additives: |
None |
| Specific activity: |
>3,000 units/mg (Turbidometric assay AMBI Protocol TP-031) |
| Physical form: |
Lyophilized powder |
| Shelf life: |
<1% loss of activity per year, when stored dry at -80° C |
| |
| Batch Information |
|
| Lot Number: |
GDV 1 |
| Specific activity: |
3552 units/mg |
| Peptide purity: |
93.4% (HPLC, % of peak area) |
| |
| References |
|
| Recsei, P.A. (June 5, 1990) U.S. Pat. # 4931390. |
| Recsei, P.A., Gruss, A.D., and Novick, R.P. (1987) Cloning, sequence, and expression of
the lysostaphin gene from Staphylococcus simulans. Proc. Nat. Acad. Sci., U.S.A. 84: 1127-1131. |
| Kline, S.A., J. de la Harpe, and P. Blackburn. (1994). A colorimetric microtitre plate assay for lysostaphin using a hexaglycine substrate. Anal. Bioch. 217, 329-331. |
| Schindler, C.A. and V.T. Schuhardt. (1964) Lysostaphin: a new bacteriolytic agent for the stapylococci.Proc. Nat. Acad. Sci. U.S.A. 51: 414-421. |
| Novick, R. P. (1991). Methods in Enzymology 204:587-636 |
| Goering , R.V and M. A. Winters. (1992). Rapid method for epidemiological evaluation of Gram-positive cocci by field inversion gel electrophoresis. J. Clin. Microbiol. 30:577-580 |
| Bannerman, T. L., G. A. Hancock, F. C. Tenover and J. M. Miller. (1995).
Pulsed-field gel electrophoresis as a replacement for bacteriophage typing of Staphylococcus aureus. J. Clinical Microbiol. 33:551-555 |
